The non-ionic detergent Brij 58P mimics chaperone effects

Fractionation of proteins of the microsomes of rat liver by means of a non-ionic detergent.

reasonable to suppose that the ratio k(Gly. Gly)/ kl(Leu. Gly) gives an approximate estimate of the relative selectivities of the reagents towards the splitting of peptide bonds. A simple calculation from the results in Table 2 gives a value of the ratio of 5 for aqueous hydrochloric acid and average values of 16 for methylhydrazine and 22 for hydrazine. Therefore the selectivity of the reagent...

Non-ionic detergent affects the conformation of a functionally active mutant of Bcl-X(L).

We found that a mutant, Bcl-X(L)(F131V), which was previously reported to have impaired binding capacity, can bind to Bax almost as strongly as wild-type Bcl-X(L). In the absence of detergent, the Bcl-X(L)(F131V) mutant adopts the same conformation as wild-type Bcl-X(L), as determined by circular dichroism spectroscopy and size-exclusion chromatography. However, non-ionic detergent induces a co...

CMPE 58P Notes (Draft)

Shape transformation and burst of giant POPC unilamellar liposomes modulated by non-ionic detergent C12E8.

We studied spontaneous shape transformations and burst of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC) vesicles with exogeneously added non-ionic detergent octaethylene-glycol dodecylether C(12)E(8). The addition of C(12)E(8) increased the speed of the vesicle shape transformation, so that we were able to study for the first time the complete sequence of POPC vesicle shapes star...

Characteristics of n-octyl beta-D-thioglucopyranoside, a new non-ionic detergent useful for membrane biochemistry.

n-Octyl beta-D-thioglucopyranoside, a new non-ionic detergent, was synthesized. Properties, and applicability to membrane proteins, of this detergent were investigated. The detergent was easily removed by dialysis. The solubilizing power of this detergent for Escherichia coli membrane proteins was similar to that of n-octyl beta-D-glucopyranoside, which has been widely used in membrane biochemi...